Analytical Techniques In Biochemistry

Offered bySwayam

Analytical Techniques In Biochemistry
at
Swayam
Overview

Comprehensive Guide to Essential Biochemical Analysis Techniques for Research, Diagnostics, and Industry Applications

Duration	15 weeks
Start from	Start Now
Mode of learning	Online
Difficulty level	Beginner
Official Website	Go to Website
Credential	Certificate

Analytical Techniques In Biochemistry
at
Swayam
Highlights

Earn a certification after completion
Learn from expert faculty

Analytical Techniques In Biochemistry
at
Swayam
Course details

Skills you will learn

SEM

Who should do this course?

Individuals working in clinical, research, and industrial laboratories who perform biochemical analyses

What are the course deliverables?

Describe the basic principles and theoretical background of key biochemical analytical techniques
Explain the biochemical and molecular basis of the analytical methods used in modern biochemistry
Demonstrate the ability to perform common biochemical assays and techniques, such as spectrophotometry, chromatography, electrophoresis, and mass spectrometry
Operate and troubleshoot standard biochemical laboratory equipment and instruments

More about this course

The analytical techniques routinely used in Biochemistry take a very important place in Biochemistry, learning them is not just a requirement but, absolutely essential. This is because, the understanding of the subject mainly comes from the strong basis of the experiments and techniques on which the theories are built
This course deals with various techniques their principles, experimental details etc., including general Laboratory techniques, separation techniques based on hydrodynamic properties, centrifugation (sub-cellular fractionation etc), and Chromatographic, and Electrophortic techniques , HPLC, Purification methods, ELISA etc
Application in biomedical research and some clinical diagnostic methods RIA, ELISA, PET etc., Moleular diagnostics ,PCR, etc., Microscopic techniques and clinical biochemistry

Analytical Techniques In Biochemistry
at
Swayam
Curriculum

Week 1

Aqueous solutions , Acids, Bases, buffers systems and pH meter,Colorimetry and UV-VIS absorption spectroscopy,Introduction to Hydrodynamic Techniques

Week 2

Centrifugation: Principles & Methodology,Density Gradient centrifugation : Rate Zonal and isopycnic,Differential centrifugation for Sub-cellular Fractionation

Week 3

Gel filtration: Principle, Methodology & Applications,Planar Chromatography: Principles and Applications

Week 4

Ion-exchange chromatography,Affinity chromatography

Week 5

Gas chromatography,HPLC,Protein Estimation Techniques

Week 6

Assessment-2,Light Microscopy and Confocal Microscopy,Fluorescence Microscopy : Application to live cell imaging

Week 7

IHC and IF,Flow cytometry: Theory and concept,Flow cytometry:Application in biology and medicine

Week 8

Electron Microscopy, TEM and SEM,SDS-PAGE

Week 9

Western Blotting Techniques,2D Electrophoresis and DIGE,Principles of Mass spectrometry

Week 10

Clinical Proteomics,Methods to quantify and Integrity check of DNA

Week 11

Methods to quantify and Integrity check of RNA,PCR and Real Time PCR,Blotting techniques for Nucleic acids

Week 12

Sequencing techniques of nucleic acids,Introduction to Recombinant DNA Technology,Assessment-2

Analytical Techniques In Biochemistry
at
Swayam
Faculty details

Prof. M.R Rajeswari

Dr. MOGANTY R. RAJESWARI obtained her M.Sc. (Chem.) from Andhra University and M.Phil. and Ph.D. (Chem.) from University of Delhi. She has 30 years of teaching and 37 yrs of research experience. She supervised 44 thesis of Ph.D., M.D., M.D.S., M.Sc., M. Biotech.