UNIGE - Chemical Biology
- Offered byCoursera
Chemical Biology at Coursera Overview
Duration | 21 hours |
Total fee | Free |
Mode of learning | Online |
Difficulty level | Intermediate |
Official Website | Explore Free Course  |
Credential | Certificate |
Chemical Biology at Coursera Highlights
- Shareable Certificate Earn a Certificate upon completion
- 100% online Start instantly and learn at your own schedule.
- Flexible deadlines Reset deadlines in accordance to your schedule.
- Intermediate Level Basic training in chemistry biology and physics.
- Approx. 21 hours to complete
- English Subtitles: French, Portuguese (European), Russian, English, Spanish
Chemical Biology at Coursera Course details
- Chemical biology is a burgeoning field that has rapidly risen to prominence. This surge of interest has been fuelled by chemical biology?s applicability to understanding critical processes in live cells or model organisms in real time. This success has arisen because chemical biology straddles a nexus between chemistry, biology, and physics. Thus, chemical biology can harness rapid chemistry to observe or perturb biological processes, that are in turn reported using physical assays, all in an otherwise unperturbed living entity.
- Although its boundaries are endless, the multidisciplinary nature of chemical biology can make the field seem daunting; we beg to differ! Here, we deconstruct chemical biology into its core components, and repackage the material. In the process we build up for each student a practical and theoretical knowledge bank that will set these students on their way to understanding and designing their own chemical biology experiments.
- We will discuss fluorescence as a general language used to read out biological phenomena as diverse as protein localization, membrane tension, surface phenomena, and enzyme activity. We will proceed to discuss protein labeling strategies and fusion protein design. Then we will discuss larger and larger scale chemical biology mechanism and screening efforts. Highlights include a large amount of new data, tailored in the lab videos, and a large number of skilled presenters.
Chemical Biology at Coursera Curriculum
Welcome to Chemical Biology: Concepts and techniques
Trailer
Welcome
Why study chemical biology?
Course outline and highlights
The building blocks: monomeric units of proteins
The building blocks: monomeric units of DNA and membranes
Messengers and messages: posttranslational modifications, and pathways
Studying cellular processes with chemical biology in real time
Course Acknowledgements
How assessments work
The foundation: the cell
The workhorses: proteins, enzymes
Classical protein readouts
Flash mob: overview of protein analysis methods
Flash mob - Chemistry
Components of a cell
Amino acids
Match technique to goal
Final Quiz Number 1
The Devil is in the Minutest Detail
Welcome to Module 2
Light'em up! Fluorophores: structures, and fluorescent principles
Get out (almost) what you put in!
Traditional light microscopy ? applications
Traditional light microscopy ? precautions
Physics of fluorescence
Fluorescent proteins
RNA interference and genome editing
Plasmids and transfection
DNA double stranded breaks and ?-H2AX
Jablonski diagram
Match color of fluorophore to structure
Final Quiz Number 2
A ruler over time and space! Fluorescent assays to measure complex parameters in real time
Welcome to Module 3
Creating modified chromatin
PRC2, a DNA binding complex linked to development and cancer
TIRF talk
On our TIRF
If your protein surfs on DNA, hit the TIRF to study it
PHF1 Prolongs the stable PRC2-DNA complex, promoting activity
Fluid mosaic model
Don?t get uptight! New methods to measure membrane tension
Getting a grip on membrane tension measurements
TORCing TORC: the many faces of the TOR proteins
TORCing it out: pathways that seek to sense and restore membrane tension
DNA organization in eukaryotes is linked to regulation
Kinetics of chemical reactions
Comparing chemical structures
Flash mob inteins
Model Organisms
Making proteins via expressed protein ligation and alkylation
Hit the tirf: applications of TIRF microscopy
Analyzing data from TIRF experiments to gain mechanistic insights
Modern fluorescence methods, the spark of creativity
TORCing about Tor's role in membrane tension regulation
Final Quiz 3
Putting proteins to work for us
Welcome to Module 4
Tracking a specific protein in live cells & in real time
Molecular zipcodes
How to design your fusion protein
Design principle and optimization of SNIFITs
SNIFITs to ID neurotransmitters and drugs
Application to point of care diagnosis
Biological electrophiles, the cell?s way of saying ?I?m stressed??
Breaking the tether is not that bad
T-REX: a question of control(s)
T-REX rewiring of kinase activity through targeting AKT and PTEN: opposing forces meet
Many enzyme mechanisms proceed through a covalent enzyme intermediate that can be stabilized through mutagenesis
Small molecule signals
Weighing up our options
Optimal considerations for making fusion proteins and employing them in chemical biology
Optimal considerations for making fusion proteins and employing them in chemical biology2
A bit on the nose? Chose the right SNIFITs
Final Quiz Number 4
Making light work of it on a larger and larger scale
Welcome to Module 5
A fat lot of use? Proteins that bind and transport lipids
Greasing the wheels of discovery for new interactomes
Laser guided lipids
Deuterium labeling to assess how sphingosine originating in lysosomes is metabolized
Deuterium labeling and knockout combined
Mitochondria-released sphingosine does not elicit calcium signaling: global sphingosine release does
G-REX ? a method to release specific electrophiles on demand
G-REX identifies important novel signaling proteins that function in fish
Sphingolipids
Endogenous electrophile signaling
To cage or not to cage?
interpretation of pulse chase data from wild type and knockdown lines
Variables G-REX controls that traditional methods do not
Final Quiz Number 5
Casting wide your net
Welcome to Module 6
Peptide Nucleic Acid ? Use in library synthesis and split and mix approach
In the lab - split and mix approach in a nutshell
PNA libraries show their MITE
It takes two: library diversification through DNA display
A STING operation: inhibiting a protein implicated in numerous inflammatory diseases
Performing the Screen
Mutagenesis: ensuring we stay on target
The more, the merrier: Phage display, necessity, strengths and weaknesses
Workflow for split and mix
Different methods to achieve diversity
components and controls for Luciferase assay
Final Quiz Number 6
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