IIT Delhi develops way to detect Omicron in 90 minutes

Currently, the identification or screening for Omicron variant of Covid is done worldwide using next-generation sequencing-based methods, which require over three days. 

Researchers at the Indian Institute of Technology (IIT) Delhi’s Kusuma School of Biological Sciences have developed an RT-PCR based assay for the specific detection of the Omicron (B.1.1.529.1) variant of SARS-CoV-2.  

Currently, the identification or screening for Omicron is done worldwide using next-generation sequencing-based methods, which require over three days. By using this RT-PCR based assay, it will be possible to test for the presence of the Omicron variant within 90 minutes. 

The assay is based on detecting specific mutations, which are present in the Omicron variant and absent in other currently circulating variants of SARS-CoV-2. 

Primer sets targeting these unique mutations in the S gene were designed for the specific amplification of either the Omicron variant or other currently circulating variants of SARS-CoV-2 and tested using real time PCR. 

Using synthetic DNA fragments, the assays were optimised to distinguish the wild-type from the Omicron variant in a dynamic range from 107 to <100 copies/reaction. 

This can be used as a rapid screening assay for the identification and isolation of individuals with the Omicron variants. IIT Delhi has filed an Indian patent application for the same and is in the process of initiating talks with potential industry partners. 

IIT Delhi  had earlier obtained approval from  the Indian  Council of Medical Research  ((ICMR),  the  first  academic Institute in India to do so))  for an RT-PCR kit for the diagnosis of COVID,  which was successfully launched in the market.   

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